次世代高通量定序專題演講: SMRT Sequencing solutions for plant and animal genome research

講題：SMRT® Sequencing Solutions for Plant and Animal Genome Research
講者：Deryi Mu, Sales Director for North Asia, Pacific Biosciences
時間：2015/04/09 14:00-15:00
地點：台灣大學生命科學院三樓 332 演講廳

演講摘要：
PacBio® Single Molecule, Real-Time (SMRT) Sequencing holds promise for addressing new frontiers in large genome complexities, such as long, highly repetitive, low-complexity regions and duplication events that are difficult to resolve with short-read technologies. We present solutions available for plant and animal genome research to best leverage long reads that have exceeded 20 Kb in length. Benefits for these applications are further realized with consistent use of size-selection of input sample using the BluePippin™ device from Sage Science. Highlights from our genome improvement projects using the latest P6-C4 chemistry on model organisms demonstrate contig N50 exceeding 6 Mb and longest contig exceeding 12.5 Mb with an average base quality of QV50. Targeted solutions can also be employed to conduct gene surveys and specific characterizations to understand biodiversity of traits. Our Iso-Seq™ Application offers direct sequencing of full-length transcripts to understand isoform diversity and improve annotations of genomes.